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Specialty Columns

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There are several million compounds isolated and described in chemical literature. An even greater number of combinations of these compounds (mixtures) requires analysis by HPLC. SIELC produces custom phases for our customers who were not able to obtain desirable results with commercially available HPLC phases.

These new phases are optimized to provide either best selectivity, shorter run time, desirable mobile phase composition, or a combination of all of these. Whatever the challenge you face in the real world analytical situation, the optimum separation media can be developed to get the best and usually most economical separation performance.

Some of these custom made phases have turned out to be useful in multiple situations and can be used by more than one customer. Some of the custom made materials are now available as specialty columns.

These columns go through the standard strict testing, packing and performance optimization. The material used to pack specialty columns is available in bulk quantity, with particle sizes different from the typical analytical 5 and 10 um sizes.

PEI specific column was designed to combine ion-exclusion and size-exclusion phenomena to allow for separation of
PEI polymers from most other higher and lower-molecular-weight compounds and excess of Cu (II) ions.

Separates PEI polymers

Primesep S2 is a silica-based acidic-column with hydrophilic properties.

Separates basic compounds by cation-exchange mechanism
Separates basic and neutral polar compounds by HILIC mechanism
All mobile phases are LC-MS and preparative chromatography compatible

Primesep B4 is a reverse-phase (short carbon chain) analytical column with embedded basic ion-pairing groups.

Improves retention of acidic compounds by anion-exchange mechanism
Retains and separates basic and acidic surfactants based on multi-mode mechanism
Separates hydrophobic bases by ion-exclusion mechanism
Retains hydrophobic neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 4.5)

Primesep PB is a reverse-phase analytical column with embedded aromatic and basic ion-pairing groups.

Improves retention of acidic compounds by anion-exchange mechanism
Separates aromatic compounds by difference in pi-pi interaction
Retains neutral compounds by reverse-phase mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 5)

Primesep S is a normal-phase analytical column with embedded acidic ion-pairing groups.

Most suitable for separation of common sugars and other neutral very polar molecules
Retains and separates amino acids with different buffers at low concentration
Separates weak bases by ion-exchange mechanism
Separates acids by ion-exclusion mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 7.0)

Primesep AP is a silica-based amino-column with hydrophilic properties.

Separates acidic compounds by anion-exchange mechanism
Separates basic and neutral polar compounds by HILIC mechanism
All mobile phases are LC-MS and preparative chromatography compatible (working pH range from 2.0 to 7.0)

Primesep X is a silica-based amino-column with weak hydrophobic properties.

Separates acidic compounds by anion-exchange mechanism
Separates basic and neutral compounds by RP mechanism
All mobile phases are LC-MS and preparative chromatography compatible

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